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Journal: PLoS ONE
Article Title: The Transcriptomics of Glucocorticoid Receptor Signaling in Developing Zebrafish
doi: 10.1371/journal.pone.0080726
Figure Lengend Snippet: Of 12261 potential unique genes, the mRNA expression of 1313 were found to be statistically significantly changed at 24 hpf, with 583 downregulated (grey) and 730 upregulated (black). 836 genes were changed with statistical significance at 36 hpf, of which 243 were downregulated and 593 were upregulated. (n=3 pools of 25 embryos used for microarray analysis, P≤0.05, Students t -test with Benjamini-Hochberg false-discovery rate correction).
Article Snippet: To better understand of the molecular mechanisms involved, we investigated changes in the developmental transcriptome prior to hatch, in response to morpholino oligonucleotide knockdown of GR using the
Techniques: Expressing, Microarray
Journal: PLoS ONE
Article Title: The Transcriptomics of Glucocorticoid Receptor Signaling in Developing Zebrafish
doi: 10.1371/journal.pone.0080726
Figure Lengend Snippet: qPCR analysis was performed on 7 genes to confirm the transcript abundance seen with the microarray analysis. The selected genes were bmp7a (A), f5 (B) , ff1d (C), myom1a (D), pomca (E), star (F), mc1r (G). Data is presented as mean ± standard error of the mean (normalized to β-actin, SEM; n=5-7 pools of 25 embryos each); * denotes statistical significance ( t -test, p<0.05). (See for fold-changes and p-values).
Article Snippet: To better understand of the molecular mechanisms involved, we investigated changes in the developmental transcriptome prior to hatch, in response to morpholino oligonucleotide knockdown of GR using the
Techniques: Microarray
Journal: PLoS ONE
Article Title: The Transcriptomics of Glucocorticoid Receptor Signaling in Developing Zebrafish
doi: 10.1371/journal.pone.0080726
Figure Lengend Snippet: List of genes confirmed using microarray and qPCR with fold-changes and p-values.
Article Snippet: To better understand of the molecular mechanisms involved, we investigated changes in the developmental transcriptome prior to hatch, in response to morpholino oligonucleotide knockdown of GR using the
Techniques: Microarray